Invitrogen™ MultiShot™ FlexPlate Mach1™ T1^R Competent Cells

Description

Includes

Box 1:

• MultiShot™ FlexPlate Mach1™ T1R Chemically Competent E. coli
• One 96-well plate, 20 μl per well

Box 2:

• pUC19 DNA (10 pg/μl): 1 vial, 50 μl
• S.O.C. medium: 2 bottles, 10 ml each

MultiShot FlexPlate Mach1 T1^R chemically competent E. coli cells are exceptionally fast-growing cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

MultiShot FlexPlate Mach1 T1^R chemically competent E. coli cells are exceptionally fast-growing cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity. These chemically competent cells are well suited to reduce the time required for clone evaluation. The convenient FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.

All of our T1^R strains have the tonA (also known as fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Accelerate cloning workflow using Mach1 T1^R strain
MultiShot FlexPlate Mach1 T1^R competent E. coli cells are fast growing chemically competent cells with a doubling time of approximately 50 minutes. Colonies are clearly visible within 8 hours of plating the transformation mix (ampicillin selection), enabling for plating and selecting of colonies the same day. Plasmid DNA may also be prepared in four hours after inoculating a single, overnight-grown colony in the selective media of choice (with any antibiotic).

MultiShot FlexPlate Mach1 T1^R Competent Cells offer:
• Transformation efficiencies of >1 x 10⁸ cfu/μg plasmid DNA
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• lacZ∆M15 for blue-white color screening of recombinants
• hsdR mutation for efficient transformation of unmethylated DNA from PCR applications
• recA1398 mutation for reduced occurrence of homologous recombination in cloned DNA
• endA1 mutation for increased plasmid yield and quality

Increase transformation productivity with MultiShot FlexPlate format
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler.

Key benefits of the MultiShot FlexPlate format:
• Increase throughput—compatible with automated liquid handling platforms
• Flexible—transform in increments of 8 wells to entire 96-well plate
• Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype: F^– φ80lacZΔM15 ΔlacX74 hsdR(r_K–, m_K+) ΔrecA1398 endA1 tonA

Find the strain and format that you need
• A variety of E. coli strains and formats of Chemically Competent cells and Electrocompetent cells are available to meet specific transformation needs.
• We offer bacterial growth media in various formats.
• For high-throughput transformation options, choose from our collection of MultiShot formatted comp cells.

Specifications

• Product Type: Chemically Competent Cells
• Contains F' Episome: No
• Improves Plasmid Quality: Yes (endA1)
• Cloning Methylated DNA: No
• Content And Storage: • MultiShot FlexPlate Mach1 T1^R Competent Cells (1 x 96-well plate at 20 μL/well); store at –80°C
  • pUC19 DNA (50 μL at 10 pg/μL); store at –20°C.
  • S.O.C. Medium (2 x 10 mL); store at 4°C or room temperature
  
  
• Antibiotic Resistance Bacterial: No
• Blue/White Screening: Yes (lacZΔM15)
• High-throughput Compatibility: High
• Preparing Unmethylated DNA: No
• Reduces Recombination: Yes (recA1)
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): Yes
• Species: E. coli (wild-type W)
• Format: 96-well Plate
• Product Line: Multishot™
• Quantity: 1 x 96 well plate (20 μl/well)

For Research Use Only. Not for use in diagnostic procedures.