Invitrogen™ MultiShot™ FlexPlate OmniMAX™ 2 T1^R Competent Cells

Description

Includes

Box 1:

• MultiShot™ FlexPlate OmniMAX™ 2 T1R Chemically Competent E. coli
• One 96-well plate, 20 μl per well

Box 2:

• pUC19 DNA (10 pg/μl): 1 vial, 50 μl
• S.O.C. medium: 2 bottles, 10 ml each

MultiShot FlexPlate OmniMAX 2 T1^R chemically competent E. coli cells are a superior strain of cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

MultiShot FlexPlate OmniMAX 2 T1^R chemically competent E. coli cells are a superior strain of cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.

All of our T1^R strains have the tonA (also known as fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.
 
Achieve highest transformation efficiency with OmniMAX 2 T1^R chemically competent cells
The MultiShot FlexPlate OmniMAX 2 T1^R E. coli strain is an improved chemically competent cell line that provides transformation efficiencies in the MultiShot FlexPlate format of >5 x 10⁸ cfu/μg with control plasmid DNA. It is versatile strain, perfect to use in all cloning applications, including with Gateway technology and TOPO PCR cloning. The OmniMAX 2 T1^R strain lacks the E. coli K12 restriction systems (mcrA Δ(mrr hsdRMS-mcrBC)) allowing efficient transformation of highly methylated DNA. The OmniMAX 2 T1^R strain has the lacZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. It also carries the recA1 mutation that helps reduce the rate of recombination while propagating plasmid DNA, and the endA1 mutation that increases plasmid DNA yield and quantity. The OmniMAX 2 T1^R strain contains an F´ episome that can support cloning plasmids with an f1-like origin of replication (phagemids). In addition, the F´ episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG. Importantly, the ccdA gene present on the F´ episome is deleted, allowing negative selection of Gateway vectors since the strain is sensitive to the ccdB gene product.

One Shot OmniMAX 2 T1^R Chemically Competent E. coli offer: 
• Highest transformation efficiency in FlexPlate format of >5x10⁸ cfu/μg control DNA
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• Δ(ccdAB) for sensitivity to toxic effects of ccdB gene product, allowing negative selection of vectors containing ccdB gene (Gateway cloning system)
• Stable F´ episome that carries lacIq repressor for inducible expression from trc, tac, and lac promoters
• Construction of more representative genomic libraries due to the elimination of mcrA, mcrBC, mrr, and hsdRMS
• lacZΔM15 for blue-white color screening of recombinant clones
• endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Increase transformation productivity with MultiShot FlexPlate format
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler.

Key benefits of the MultiShot FlexPlate format: 
• Increase throughput—compatible with automated liquid handling platforms
• Flexible—transform in increments of 8 wells to entire 96-well plate
• Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype
F´ [proAB+lacIqlacZΔM15 Tn10(TetR) Δ(ccdAB)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 (NalR) relA1 tonA panD

Find the strain and format that you need
• We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs.
• If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells.
• We offer bacterial growth media in various formats.

Specifications

• Product Type: Chemically Competent Cells
• Contains F' Episome: Yes
• Improves Plasmid Quality: Yes (endA1)
• Cloning Methylated DNA: Yes (mcrA)
• Content And Storage: • MultiShot FlexPlate OmniMAX 2 T1^R Competent Cells (1 x 96-well plate at 20 μL/well)
  Store at –80°C.
  
  • pUC19 DNA (50 μL at 10 pg/μL)
  Store at –20°C.
  
  • S.O.C. Medium (2 x 10 mL)
  Store at 4°C or room temperature.
  
  
• Antibiotic Resistance Bacterial: Yes (Tetracycline)
• Blue/White Screening: Yes (lacZΔM15)
• High-throughput Compatibility: High
• Plasmid: May be used for plasmids > 20 kb
• Preparing Unmethylated DNA: No
• Reduces Recombination: Yes (recA1)
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): Yes
• Species: E. coli (K12)
• Format: 96-well Plate
• Product Line: Multishot™
• Quantity: 1 x 96 well plate (20 μl/well)

For Research Use Only. Not for use in diagnostic procedures.