Invitrogen™ MultiShot™ StripWell DH5α T1^R Competent Cells

Description

Includes

Box 1:

• MultiShot StripWell DH5α T1R Chemically Competent E. coli
• One rack of 12 strips x 8-tubes, 50 μl per tube

Box 2:

• pUC19 DNA (10 pg/μl): 1 vial, 50 μl
• S.O.C. medium: 2 bottles, 15ml each

MultiShot StripWell DH5α T1^R chemically competent E. coli cells are cloning competent cells packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations.

MultiShot StripWell DH5α T1^R chemically competent E. coli cells are cloning competent cells packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations. This strain is derived from the popular DH5α strain, one of the most frequently used laboratory cloning strains. All of our T1^R strains have the tonA (also known as fhuA) mutation in their genotype that confers resistance to T1 and T5 phages. A resistant genotype offers added security to protect valuable clones and libraries as T1 bacteriophage can rapidly spread among E. coli hosts and contaminate repositories.

DH5α T1^R for routine cloning applications
The DH5α strain is well-known and broadly used laboratory strain for chemical transformations as it is ideal for transformations of cloned unmethylated DNA. To increase productivity for medium-throughput bacterial transformations, the cloning competent DH5α T1^R E. coli cells are packaged in racks containing 12 strips of 8.

MultiShot StripWell DH5α T1^R Competent Cells include:
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA
• tonA (fhuA) for T1 and T5 phage resistance 

MultiShot StripWell flexible format
Each StripWell of eight connected tubes is designed with the same single-tube, single-use features as our One Shot format tubes. This format allows all steps of the transformation protocol, up to plating, to take place in the same tube, saving time and reducing chance of contamination. After addition of DNA, MultiShot StripWell competent cells can be transformed by heat-shock at 42°C in a water bath without transfering to a new tube.

The StripWell tubes can also be cut to permit single transformations. This feature eliminates wasted aliquots and avoids needless freeze-thaw cycles that can result in reduced transformation efficiency. 

Benifits of the MultiShot StripWell format include:
• Increased productivity—suitable for high throughput experimentation 
• Maximal flexibility—transform as few as 1 tube or as many as 96 from the StripWell rack
• Familiar convenience—add DNA directly to cells, heat shock, and recover in the same tube

Genotype
F^- φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ thi-1 gyrA96 relA1 tonA

Find the strain and format that you need
• We offer a variety of DH strains in both chemically competent and electrocompetent cell formats.
• For high-throughput transformation options, choose from our collection of MultiShot Formatted Competent Cells.

Specifications

• Product Type: Chemically Competent Cells
• Contains F' Episome: No
• Cloning Methylated DNA: No
• Content And Storage: • MultiShot StripWell DH5α T1^R Competent Cells (1 x 96-well plate at 50 μL/well)
  Store at –80°C.
  
  • pUC19 DNA (50 μL at 10 pg/μL)
  Store at –20°C.
  
  • S.O.C. Medium (2 x 15 mL)
  Store at 4°C or room temperature.
  
  
• Antibiotic Resistance Bacterial: No
• Blue/White Screening: Yes (lacZΔM15)
• High-throughput Compatibility: High
• Preparing Unmethylated DNA: No
• Reduces Recombination: Yes (recA1)
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): Yes
• Species: E. coli (K12)
• Format: 8-well Strips
• Product Line: Multishot™
• Quantity: 1 x 96 tube rack (50 μl/tube)

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.