Invitrogen™ MyD88 Polyclonal Antibody, eBioscience™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 14622363

Catalog No. 50-134-71

Description

Description: The polyclonal antibody reacts with human and mouse MyD88; the antibody was raised against residues 279-296 of human MyD88 and recognizes human and mouse antigens. MyD88, a myeloid differentiation primary response gene, is expressed in a variety of tissues and functions as an adapter molecule in the IL-1 signaling pathway involved in the inflammatory responses induced by cytokines and LPS. MyD88 associates with and recruits IRAK to the IL-1 receptor. Dominant negative mutants of MyD88 attenuate IL-1R-mediated NF-kappaB activation. MyD88 also functions as a regulator molecule for IL-18 receptor and human Toll receptor family. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to LPS. Applications Reported: This polyclonal antibody has been reported for use in immunoblotting (WB). Applications Tested: This polyclonal antibody has been reported for use in immunoblotting (1:500-1:1000 dilution) of MyD88 from Jurkat cell lysate as a positive control. A 35 kDa band can be detected. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Myeloid differentiation Marker 88 (MyD88) is an adaptor molecule for the Toll/IL-1R and is involved in the inflammatory response induced by IL-1, IL-18 and LPS. MyD88 recruits IRAK to IL-1 receptor complex in response to IL-1. This pathway further leads to activation of NF-kB. MyD88 has a wide tissue distribution.

Specifications

• Antigen: MyD88
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MYD88
• Gene Alias: mutant myeloid differentiation primary response 88; Myd88; MYD88D; myeloid differentiation primary response 88; myeloid differentiation primary response gene (88); myeloid differentiation primary response gene 88; myeloid differentiation primary response protein MyD88
• Host Species: Rabbit
• Quantity: 10 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P22366, Q99836
• Gene Symbols: MYD88
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17874, 4615
• Content And Storage: 4°C
• Form: Liquid