Description: This F5D monoclonal antibody reacts with human, mouse, dog, and rat myogenin, a 34-kDa transcription factor. Expressed in skeletal and heart muscle, myogenin is a member of the MyoD family of basic-helix-loop-helix proteins, which also includes MyoD, Myf5, and MRF4. This transcription factor interacts with other helix-loop-helix proteins, which may or may not be muscle-specific. Myogenin plays a significant role in myogenic differentiation, even directing nonmuscle cells to the myogenic lineage. Transforming growth factor-beta (TGFb) and bone morphogenetic protein-2 (BMP2) inhibit myogenin transcriptional activity. Predomitly residing within the nucleus, the subcellular localization of myogenin has been shown to be dependent on differentiation status and cell density. For instance, trafficking of myogenin between the nucleus and cytoplasm has been reported during skeletal muscle differentiation to mediate transcription control. Applications Reported: This F5D antibody has been reported for use in immunoblotting (WB) and immunocytochemistry. Applications Tested: This F5D antibody has been tested by western blot and immunocytochemistry of the C2C12 cell line. This can be used at less than or equal to 1-5 µg/mL for both applications. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered. Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix proteins. It is essential for the development of functional skeletal muscle.
|Canine, Human, Mouse, Rat|
|Immunocytochemistry, Immunofluorescence, Western Blot|
|PBS with 0.09% sodium azide; pH 7.2|
For Research Use Only.
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