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  6. Invitrogen™ Myosin 4 Monoclonal Antibody (MF20), Alexa Fluor™ 488, eBioscience™, Invitrogen™ 

Invitrogen™ Myosin 4 Monoclonal Antibody (MF20), Alexa Fluor™ 488, eBioscience™, Invitrogen™
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Catalog No. 501128865
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50-112-8865 100 μg
50-112-8752 25 μg
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Catalog No. 50-112-8865 Supplier Invitrogen™ Supplier No. 53650382
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Description

Mouse Monoclonal Antibody

Description: This MF20 monoclonal antibody recognizes the heavy chain of myosin II, specificially the light meromyosin portion, in cardiac and skeletal muscle of vertebrates. Myosin II is composed of two heavy chains and four light chains. The 220-kDa myosin heavy chain exists as four different isoforms due to alternative splicing. Myosins interact with actin and hydrolyze ATP to function in muscle contraction, cytokinesis, and phagocytosis. The MF20 has been shown to react to myosin from a variety of mammalian, avian and amphibian species, including rat, mouse, human, chicken, zebrafish, and dog. Applications Reported: This MF20 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, and immunohistochemical staining of frozen tissue sections. Applications Tested: This MF20 antibody has been tested by immunocytochemistry on fixed and permeabilized C2C12 cells that were differentiated for 2 days in low serum medium prior to staining. Staining can be performed using less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Target protein includes a fast myosin isoform expressed in skeletal muscle and has function involved in Muscle contraction.
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Specifications

Antigen Myosin 4
Applications Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry
Classification Monoclonal
Clone MF20
Concentration 0.5 mg/mL
Conjugate Alexa Fluor 488
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene MYH4
Gene Accession No. Q076A5, Q28641, Q29RW1, Q5SX39, Q9Y623
Gene Alias 2B myosin heavy chain; AI506973; fast myosin heavy chain 2B; LOW QUALITY PROTEIN: myosin-4; MHC; MHC2B; Minimsc; Minmus; MM; MYH1; MYH2B; MYH-2B; myh4; MyHC IIb isoform; myHC-2b; MyHC-IIb; Myhsf; myosin heavy chain; myosin heavy chain 1; myosin heavy chain 2b; myosin heavy chain 2X; myosin heavy chain 4; Myosin heavy chain IIb; myosin heavy chain IIb isoform; myosin heavy chain isoform 2b; myosin heavy chain type IIb; Myosin heavy chain, skeletal muscle; myosin heavy chain, skeletal muscle, fetal; Myosin heavy chain, skeletal muscle, juvenile; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 4, skeletal muscle; myosin-4; unnamed protein product
Gene Symbols MYH4
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 100009548, 100125991, 100726456, 17884, 360543, 408020, 4622, 468396, 479502
Target Species Bovine, Canine, Chicken, Chimpanzee, Feline, Guinea Pig, Human, Mouse, Monkey, Rabbit, Rat
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2b κ
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