Novus Biologicals™ Nectin-1/PVRL1 Antibody Pair [HRP]

Mouse Monoclonal Antibody Pair

Manufacturer:  Novus Biologicals™ NBP2796605PLATES

Catalog No. NB010360

Description

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Nectin-1/PVRL1.

Specifications

• Assay: Sandwich ELISA
• Product Type: Antibody Pairs
• Assay Range: 5000 pg/mL
• Gene ID (Entrez): 5818
• Regulatory Status: RUO
• Target: Nectin-1/PVRL1
• Quantity: 5 Plates
• Assay Sensitivity: 78.125-5000 pg/mL
• Gene Alias: CD111, CD111 antigen, CLPED1ectodermal dysplasia 4 (Margarita Island type), ED4, Herpes virus entry mediator C, Herpesvirus entry mediator C, Herpesvirus Ig-like receptor, HIgRPVRR, HveC, HVECpoliovirus receptor-like 1, MGC142031, Nectin-1, OFC7nectin 1, poliovirus receptor-related 1 (herpesvirus entry mediator C), poliovirus receptor-related protein 1, PRR, PRR1MGC16207, PVRR1nectin-1, SK-12
• Immunoassay Kit Format: Conjugated

• Conjugate: HRP
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Nectin-1/PVRL1.
• For Use With (Application): Sandwich ELISA
• Kit Contents:
• Standard: Each vial contains 140 ng of recombinant Human Nectin-1/PVRL1.
• Capture Antibody: 1 mg/mL of mouse anti-Human Nectin-1/PVRL1 monoclonal antibody (in PBS, pH 7.4).
• Detection Antibody: 0.2 mg/mL of mouse anti-Human Nectin-1/PVRL1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Storage Requirements: Storage is content dependent.
• Host Species: Mouse
• Target Species: Human
• Gene Symbol: NECTIN1
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 78.125 pg/mL is recommended.