Invitrogen™ NEFH Monoclonal Antibody (RMdO-20)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 131300

Catalog No. 13-130-0

Description

This antibody reacts with 200 kDa subunit protein of human neurofilament. It specifically recognizes a nonphosphorylated epitope in the tail domain of NF-H. Dephosphorylation of NF-H will increase immunoreactivity with this antibody. This antibody is suitable for immunohistochemical staining of Bouin's and alcohol-fixed paraffin-embedded or frozen tissue sections. To stain, incubate 30-60 minutes at room temperature or overnight at 4°C.

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).

Specifications

• Antigen: NEFH
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% sodium azide
• Gene Accession No.: P12036, P16884, P19246
• Gene Symbols: Nefh
• Immunogen: Adult Rat neurofilaments.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Rabbit, Bovine, Hamster, Mollusca
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: ELISA, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: RMdO-20
• Conjugate: Unconjugated
• Gene: Nefh
• Gene Alias: 200 kDa neurofilament protein; CMT2CC; heavy neurofilament protein; I79_012512; intermediate filament protein; KIAA0845; LOW QUALITY PROTEIN: neurofilament heavy polypeptide; mKIAA0845; NEF3; Nefh; nefh.L; neurofilament; neurofilament 200kDa; neurofilament heavy; neurofilament heavy L homeolog; neurofilament heavy polypeptide; neurofilament heavy polypeptide-like; neurofilament triplet H protein; neurofilament, heavy polypeptide; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide L homeolog; neurofilament-H; NF 200 kD; NF200; Nfh; NF-H; NHC; XELAEV_18007418mg
• Host Species: Mouse
• Purification Method: Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 100357177, 100753127, 101838568, 24587, 380684, 4744, 528842
• Content And Storage: -20°C
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov