Invitrogen™ NEFL Monoclonal Antibody (DA2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA12010

Catalog No. MA1-2010

Description

MA1-2010 detects the neurofilament, light chain in human, rat, and mouse samples. MA1-2010 has been successfully used in western blot, immunohistochemistry, and immunofluorescence. By western blot this antibody detects a ~70 kDa protein representing the neurofilament, light chain in mouse brain tissue lysate. In immunohistochemistry procedures MA1-2010 recognizes the neurofilament, light chain in mouse brain tissue. In immunofluorescence procedures MA1-2010 recognizes the neurofilament, light chain in rat cerebral cortices. The MA1-2010 immunogen is enzymatically dephosphorylated porcine neurofilaments.

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

Specifications

• Antigen: NEFL
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P02547, P07196, P08551, P19527
• Gene Symbols: NEFL
• Immunogen: Enzymatically dephosphorylated porcine neurofilaments.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Pig
• Product Type: Antibody

• Applications: ELISA, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: DA2
• Conjugate: Unconjugated
• Gene: NEFL
• Gene Alias: 68 kDa neurofilament protein; AI847934; CMT1F; CMT2E; FLJ53642; light molecular weight neurofilament protein; micro glutamic acid-rich protein; NEF3; NEFL; nefl.L; neurofilament; neurofilament 3; neurofilament L subunit; neurofilament light; neurofilament light polypeptide; neurofilament protein; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light L homeolog; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament, light polypeptide L homeolog; neurofilament-l; neurofilament-light; Nf68; NFL; N-FL; NF-L; NFM; NF-M; NLC; OTTHUMP00000196614; PPP1R110; protein phosphatase 1, regulatory subunit 110; XELAEV_18018673mg; XNF-L
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100521224, 18039, 4747, 83613
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid