MilliporeSigma™ NFκB p65 EZ-TFA Transcription Factor Assay

Catalog No.	Quantity
70-620	1 Plate

Catalog No. 70-620 Supplier MilliporeSigma™ Supplier No. 70620

Description

Includes

• Primary Antibody
• NFκB Capture Probe
• NFκB Specific Competitor Oligonucleotide
• TFA Negative Control Probe
• HeLa Whole Cell Extract, TNFα Treated
• EZ-TFA (Universal Transcription Factor Assay, Chemiluminescent)

The transcription factor NFkB is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response

• Provided in a 96-well format
• When the Capture Probe, a double stranded biotinylated oligonucleotide is incubated together, the active form of NFkB contained in the nuclear extract binds to its consensus sequence
• After incubation, the extract/probe/buffer mixture is then directly transferred to the streptavidin coated plate
• The biotinylated double stranded oligonucleotide bound by active NFkB protein is immobilized and any inactive, unbound material is washed away
• The bound NFkB transcription factor subunits, p50 and p65, are detected with specific primary antibodies, a Rabbit anti-NFkB p50 (active form) and a Rabbit anti-NFkB p65
• An HRP-conjugated secondary antibody is then used for detection and provides sensitive colorimetric detection that can be read in a spectrophotometric plate reader

Specifications

• Content And Storage:
  • Store kit components at the temperatures indicated on the labels and insert until their expiration date
  • Store HeLa Whole Cell Extract at -80°C. Avoid repeated freeze-thaw cycles. Stable at -80°C for 6 months
• Detection Method: Chemiluminescence
• For Use With (Application): Affinity Binding Assay, ELISA, Activity Assay
• Quantity: 1 Plate