Invitrogen™ Rat NGF beta-Cell Lysates ELISA Kit

ELISA

Supplier:  Invitrogen™ ERNGFCLX10

Catalog No. 50-254-5336

Description

Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.

Specifications

• Accession Number: P25427
• Assay Sensitivity: 15 pg/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: NGF
• Intraassay CV: <10
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hrs 20 mins

• Assay Range: 20.58 to 15,000 pg/mL
• Conjugate: HRP
• Sample Type: Cell Lysates
• Gene ID (Entrez): 310738
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Cell Lysate 20 μL
• Target: NGF
• Total Assay Time: 4 hrs 45 mins