Invitrogen™ NGF Polyclonal Antibody

Catalog No.	Quantity
PIPA595436	100 μg

Catalog No. PIPA595436 Supplier Invitrogen™ Supplier No. PA595436

Description

Rabbit Polyclonal Antibody

Human NGF/NGF Beta shares 92.4% amino acid (aa) sequence identity with rat NGF/NGF Beta. Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.

Specifications

• Antigen: NGF
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 4mg trehalose and 0.05mg sodium azide
• Gene: NGF
• Gene Accession No.: P01138
• Gene Alias: B nerve growth factor; beta ngf; beta-nerve growth factor; BetaNGF; beta-NGF; bNGF; b-NGF; HSAN5; MGC161426; MGC161428; Nerve growth factor; nerve growth factor (beta polypeptide); nerve growth factor, beta; nerve growth factor, beta polypeptide; nerve growth factor, beta subunit; NGF; NGF b; NGF β; Ngfb; NGFbeta; NGFβ; β nerve growth factor; β NGF; βNGF
• Gene Symbols: NGF
• Host Species: Rabbit
• Immunogen: E.coli-derived human NGF/NGF Beta recombinant protein (Position: S122-A241).
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 4803
• Target Species: Human
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG

Safety and Handling

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