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Cytiva Ni Sepharose™ 6 Fast Flow
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Quantity:
5 mL
25 mL
100 mL
500 mL
Description
Ni Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.
- Designed for scaling up purification of histidine-tagged proteins.
- High protein binding capacity and minimal leakage of Ni2+ ions.
- As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- Available in convenient prepacked HisPrep and HisTrap column formats.
- Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins.
- The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity.
- Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
- As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Specifications
Specifications
Buffer | 0.1M HCl, 0.1M NaOH, 8M Urea |
Product Type | Ni Sepharose 6 FF chromatography media |
Quantity | 100 mL |
Height (English) | 9.84 in. |
Height (Metric) | 250mm |
Flow Rate | 600 cm/hr. |
Max. Pressure | 1 bar |
pH Range | 3 to 12 |
Particle Size | 90μm |
Support Type | 6% Cross-linked Agarose |
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