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  6. Cytiva Ni Sepharose™ 6 Fast Flow

Cytiva Ni Sepharose™ 6 Fast Flow

Catalog No. 45002985 Shop All Cytiva Products
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45-002-985 100 mL
45-002-987 5 mL
45-002-984 25 mL
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Catalog No. 45-002-985 Supplier Cytiva Supplier No. 17531802
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Description

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

Ni Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

  • Designed for scaling up purification of histidine-tagged proteins.
  • High protein binding capacity and minimal leakage of Ni2+ ions.
  • As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in convenient prepacked HisPrep and HisTrap column formats.
  • Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins.
  • The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity.
  • Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
  • As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

TRUSTED_SUSTAINABILITY

Specifications

Buffer 0.1M HCl, 0.1M NaOH, 8M Urea
Product Type Ni Sepharose 6 FF chromatography media
Quantity 100 mL
Height (English) 9.84 in.
Height (Metric) 250mm
Flow Rate 600 cm/hr.
Max. Pressure 1 bar
pH Range 3 to 12
Particle Size 90μm
Support Type 6% Cross-linked Agarose
Content And Storage 4° to 30°C
Matrix Ni-Sepharose
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