Thermo Scientific™ Nitrocellulose Membranes, 0.45 μm

Catalog No.	Dimensions (LxW)	Format	Sufficient For
PI88025	8 x 8 cm	Sheet	15 Mini-gel Blots
PI88014	10.5 x 7.9 cm	Sheet	15 Mini-gel Blots
PI77010	12 x 8 cm	Sheet	25 Mini-gel Blots
PI88018	3.5 m x 30 cm	Roll	≥120 Mini-gel Blots (When cut to 8 x 10 cm)

Catalog No. PI88025 Supplier Thermo Scientific™ Supplier No. 88025

Description

Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods).

Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods). The 0.45-μm pore size is well-suited for a wide range of western blotting conditions and ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp).

Features include:
• 100% pure nitrocellulose membrane
• Convenient—save time with pre-cut sheets or choose the roll format for the flexibility to cut to the ideal size
• Compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• High-binding affinity—provides excellent protein binding, blocks easily, and does not require pre-wetting with alcohol
• Low background: produce high-quality data with low background signal in both chemiluminescent and fluorescent western blotting

Specifications

• Length (Metric): 8 cm
• Width (Metric): 8 cm
• Description: Nitrocellulose Membranes, 0.45 μm, 8 x 8 cm
• Binding Capacity: ∼45–60 μg/cm² of BSA
• Quantity: 15 Pre-cut Membranes
• Format: Sheet
• Material: Nitrocellulose
• Pore Size: 0.45 μm
• Content And Storage: Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle.
  Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C.
• Shipping Condition: Room Temperature
• Dimensions (LxW): 8 x 8 cm
• Sufficient For: 15 Mini-gel Blots