Invitrogen™ NLK Polyclonal Antibody

Catalog No.	Quantity
PIPA591956	100 μL

Catalog No. PIPA591956 Supplier Invitrogen™ Supplier No. PA591956

Description

Rabbit Polyclonal Antibody

Immunogen sequence: LKICDFGLAR VEELDESRHM TQEVVTQYYR APEILMGSRH YSNAIDIWSV GCIFAELLGR RILFQAQSPI QQLDLITDLL GTPSLEAMRT ACEGAKAHIL RGPHKQPSLP VLYTLSSQAT HEAVHLLCRM LVFDPSKRIS AKDALAHPYL DEGRLRYHTC MCKCCFSTST GRVYTSDFEP VTNPKFDDTF EKNLSSVRQV KEIIHQFILE QQKGNRVPLC INPQSAAFKS FISSTVAQPS EMPPSPLVWE; Positive Samples: Mouse brain, Rat brain; Cellular Location: Cytoplasm, Nucleus.

NLK is a serine/threonine kinase involved in signal transduction and cellular communication. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors.

Specifications

• Antigen: NLK
• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Concentration: 1.12 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene: NLK
• Gene Accession No.: D3ZSZ3, O54949, Q9UBE8
• Gene Alias: AI194375; LAK1; nemo like kinase; nemo-like kinase; NLK; Protein LAK1; RGD1561602; serine/threonine protein kinase NLK; serine/threonine-protein kinase NLK
• Gene Symbols: NLK
• Host Species: Rabbit
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 278-527 of human NLK (NP_0573153).
• Purification Method: Affinity Chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 18099, 497961, 51701
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG