ACROBiosystems NLS-Cas12a Nuclease (CAA-L5149) is expressed from E.coli cells. It contains AA Ser 2 - His 1228 (Accession # A0A5S8WF58).

Supplier:  ACROBiosystems CAAL514950UG

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated endonuclease from Lachnospiraceae bacterium，Cas12a, also known as Cpf1, is a 1200-1500 amino-acids long monomeric protein that belongs to the CRISPR/Cas system , an adaptive immune system of prokaryotes that has now become a powerful tool for genome editing. CRISPR/Cas12a belongs the class II (type 5) of the CRISPR/Cas system that is defined by a single-subunit effector. Cas12a has recently emerged as an alternative for Cas9, due to its distinct features such as the ability to target T-rich motifs, no need for trans-activating crRNA, inducing a staggered double-strand break and potential for both RNA processing and DNA nuclease activity. In addition, Cas12a is able to process more structured pre-CRISPR/RNA(crRNA) molecules into mature crRNAs which allows the possibility to use both mature or pre-crRNA for genome editing purposes. All these features make the CRISPR/Cas12a system a valuable genome-engineering t

Catalog No. 50-225-3136