Invitrogen™ nNOS Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA3032A

Catalog No. PA3032A

Description

PA3-032A detects brain nitric oxide synthase (bNOS) from bovine, mouse, rabbit and rat tissues. This antibody does not detect endothelial NOS (eNOS) or inducible NOS (iNOS). PA3-032A has been successfully used in Western blot, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects an ~155 kDa protein representing bNOS from rat brain extract. Immunohistochemical staining of bNOS in rat brain with PA3-032A results in staining of the anterior pituitary. The PA3-032A immunogen is a synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat nNOS.

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

Specifications

• Antigen: nNOS
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NOS1
• Gene Alias: 2310005C01Rik; bNOS; bNOS1; Brain NOS; Constitutive NOS; IHPS1; NC-NOS; neuronal nitric oxide synthase; neuronal nitric oxide synthase NOS1; neuronal NOS; nitric oxidase synthase; nitric oxide synthase; nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); nitric oxide synthase 1, neuronal; nitric oxide synthase, brain; nNOS; N-NOS; nNOS; NOS type I; NO; NOS; NOS Type 1; NOS type I; Nos1; Nos-1; NOS-I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS
• Host Species: Rabbit
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Rabbit, Bovine
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: Conc. Not Determined
• Formulation: whole serum with 0.05% sodium azide
• Gene Accession No.: O19132, P29475, P29476, Q9Z0J4
• Gene Symbols: NOS1
• Immunogen: Synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat bNOS.
• Regulatory Status: RUO
• Gene ID (Entrez): 100009243, 18125, 24598, 4842, 536132
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

Safety and Handling

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