Invitrogen™ nNOS Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1033

Catalog No. PA1033

Description

PA1-033 detects nNOS from human, rat and mouse samples. PA1-033 has been successfully used in Western blot, ICC/IF and immunohistochemisty (paraffin) procedures. By Western blot, this antibody detects an ~160 kDa protein. Detects a few non-specific bands in Western blot analysis of rat and mouse brain lysates. This antibody is not recommended for MCF-7, PC-12, SK-N-C or U87-MG cells in Western blot applications. The PA1-033 immunogen is a synthetic peptide to residues C N(1411) R L R S E S I A F I E E S K(1425) of human nNOS. The peptide sequence is 100% conserved in rat, mouse, frog, and rabbit nNos proteins. This peptide (Cat. # PEP-190) is available for use in neutralization and control experiments.

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

Specifications

• Antigen: nNOS
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NOS1
• Gene Alias: 2310005C01Rik; bNOS; bNOS1; Brain NOS; Constitutive NOS; IHPS1; NC-NOS; neuronal nitric oxide synthase; neuronal nitric oxide synthase NOS1; neuronal NOS; nitric oxidase synthase; nitric oxide synthase; nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); nitric oxide synthase 1, neuronal; nitric oxide synthase, brain; nNOS; N-NOS; nNOS; NOS type I; NO; NOS; NOS Type 1; NOS type I; Nos1; Nos-1; NOS-I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18125, 24598, 4842
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P29475, P29476, Q9Z0J4
• Gene Symbols: NOS1
• Immunogen: Synthetic peptide corresponding to residues C N(1411) R L R S E S I A F I E E S K(1425) of human nNOS.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG