MilliporeSigma™ Novagen™ Benzonase™ Nuclease

Description

Used for removal of nucleic acids from recombinant proteins

• Genetically engineered endonuclease from Serratia marcescens
• Degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity
• Effective over wide range of conditions and possesses high specific activity
• Rapidly hydrolyzes nucleic acids to makes enzyme for viscosity reduction to reduce processing time and increase yields of protein
• Compatible with BugBuster™ and PopCulture™ Protein Extraction Reagents
• Can be added along with these reagents to eliminate viscosity and remove nucleic acids from E. coli extracts
• Functional between pH 6 and 10 and from 0 to 42°C and requires 1to 2mM Mg2+ for activation
• Active in presence of ionic and non-ionic detergents, reducing agents, PMSF (1mM), EDTA (1mM) and urea
• Activity is inhibited by >150mM monovalent cations, >100mM phosphate, >100mM ammonium sulfate, or >100mM guanidine HCl
• Packaging: Plastic bottle

Compliance

Compliant with FDA

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Specifications

• Concentration: 25 to 29U/μL
• For Use With (Application): Nucleic Acids Removal
• Formulation: Supplied in 50% glycerol
• pH Range: 6 to 10
• Quantity: 10 kU
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Product Type: Nuclease
• Purity or Quality Grade: >90%