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MilliporeSigma™ Novagen™ One Step RT-PCR Master Mix Kit

For gene expression studies from tissues and cells

Supplier:  MilliporeSigma™ 719783

Catalog No. 71-978-3

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  • Robust one-step, one-enzyme master mix system for easy reaction assembly
  • Eliminates risk of cross contamination associated with two-step RT-PCR protocols
  • High-temperature (60° C) for reverse transcription enhances read-through of RNA secondary structure
  • Optimized buffer conditions and antibody-mediated hot start for increased sensitivity
  • Rapid enzyme activation step (30 s) avoids damage of template RNA
  • 2 x 625μL 2X One Step RT-PCR Master Mix
  • 1 x 200μL 50 mM Mn(OAc)2
  • 1 x 1.1mL RNase Free Water
  • 1 x 50μL Primer F (10 pmol/µL, 5′-TCC ACC ACC CTG TTG CTG TA-3′)
  • 1 x 50μL Primer R (10 pmol/µL, 5′-ACC ACA GTC CAT GCC ATC AC-3′)
  • 1 x 50μL Positive Control RNA (5 x 108 copies/mL)


One Step RT-PCR Master master mix kit
50 rxn


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