MilliporeSigma™ Novagen™ One Step RT-PCR Master Mix Kit

Description

For gene expression studies from tissues and cells

• Robust one-step, one-enzyme master mix system for easy reaction assembly
• Eliminates risk of cross contamination associated with two-step RT-PCR protocols
• High-temperature (60° C) for reverse transcription enhances read-through of RNA secondary structure
• Optimized buffer conditions and antibody-mediated hot start for increased sensitivity
• Rapid enzyme activation step (30 s) avoids damage of template RNA

Components:

• 2 x 625μL 2X One Step RT-PCR Master Mix
• 1 x 200μL 50 mM Mn(OAc)2
• 1 x 1.1mL RNase Free Water
• 1 x 50μL Primer F (10 pmol/μL, 5'-TCC ACC ACC CTG TTG CTG TA-3')
• 1 x 50μL Primer R (10 pmol/μL, 5'-ACC ACA GTC CAT GCC ATC AC-3')
• 1 x 50μL Positive Control RNA (5 x 108 copies/mL)

Specifications

• Content And Storage: -20°C
• Product Type: One Step RT-PCR Master master mix kit
• Quantity: 50 rxn

For Research Use Only