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MilliporeSigma™ Novagen™ T7•Tag™ Affinity Purification Kit

Rapid immunoaffinity purification of target proteins that carry the T7•Tag sequence

Manufacturer:  MilliporeSigma™ 690253

Catalog No. 69-025-3

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  • Designed for rapid immunoaffinity purification of target proteins that carry the T7•Tag sequence
  • Purification is based on binding target proteins to T7•Tag Monoclonal Antibody that is covalently coupled to cross-linked agarose beads, washing away unbound proteins, and eluting at pH 2.2
  • Neutralization Buffer is included to limit protein exposure to low pH
  • Capacity will vary somewhat between different target proteins, but the beads are standardized to bind a minimum of 300µg T7•Tag β-galactosidase per milliliter of settled resin
  • The beads can be used in either batch or column methods and can be regenerated a minimum of five times without loss of binding activity


1 Kit
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