Invitrogen™ Human Amyloid Precursor Protein ELISA Kit

Description

ELISA

The Human Amyloid Precursor Protein (APP) ELISA quantitates Hu APP in human cerebrospinal fluid and cell lysates. The assay will exclusively recognize both natural and recombinant Hu APP. Principle of the method The Human APP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Amyloid Precursor Protein (APP) or Amyloid beta precursor protein functions as a cell surface kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. APP is important for neurite growth, neuronal adhesion and axonogenesis. APP is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms resulting from alternative splicing. Proteolytic cleavage of APP by beta- and gamma-secretases results in the generation of beta amyloid, which is the primary component of senile plaques. Senile plaques are one of the major histopathologic features of Alzheimer's disease. Abnormal regulation and processing of APP also plays a role in Down's syndrome, early onset familial Alzheimer's disease, and cerebral hemorrhage.

Specifications

• Accession Number: P05067
• Assay Range: 0.78 to 50 ng/mL
• Assay Sensitivity: <0.4 ng/mL
• Concentration: 0.78 to 50 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates, Cerebrospinal Fluid
• Substrate Type: Amyloid-Related Proteins
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 351
• Gene Symbol: AAA,ABETA,ABPP,AD1I,CTFgamma,CVAP,PN-II,PN2,APP
• Incubation Time: 4 hr.
• Interassay CV: 7.4%
• Intraassay CV: 6.3
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Marker Gene: APP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 10 μL, Cerebrospinal Fluid 10 μL
• Storage Requirements: 2°C to 8°C
• Target: Amyloid Precursor Protein
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs