Invitrogen™ Bolt™ Welcome Pack + iBlot™ 2 System

A complete protein separation-to-western blot solution by combining a Bolt Welcome Pack A with the iBlot 2 Gel Transfer Device and iBlot 2 Transfer Stack (nitrocellulose, regular size) in lieu of one box of Bolt gels

Supplier:  Invitrogen™ NW0412AIB2

Catalog No. NW0412AIB2

Description

The Bolt Welcome Pack + iBlot 2 System offers a complete protein separation-to-western blot solution by combining a Bolt Welcome Pack A with the iBlot 2 Gel Transfer Device (Cat. No. IB21001) and iBlot 2 Transfer Stack (nitrocellulose, regular size) (Cat. No. IB23001) in lieu of one box of Bolt gels.

The Bolt Welcome Pack A consists of a Mini Gel Tank plus all of the necessary Bolt™ gels and buffers you will need to get started running gels.

Contents of the Bolt Welcome Pack A:
• Mini Gel Tank (Cat. No. A25977)
• Bolt 4-12% Bis-Tris Plus gels, 10-well, 1 box (Cat. No. NW04120BOX)
• Bolt MES Running Buffer, 20X (Cat. No. B0002)
• Bolt LDS Sample Buffer, 4X (Cat. No. B0007)
• Bolt Sample Reducing Agent, 10X (Cat. No. B0009)
• SeeBlue Plus2 Pre-stained Protein Standard (Cat. No. LC5925)

About the Mini Gel Tank
The Mini Gel Tank is compatible with a variety of Novex gels, NuPAGE gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies being used.

About Bolt Bis-Tris Plus Gels
Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Similar to NuPAGE Bis-Tris gels, Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four g types and multiple well formats. Each box contains 10 gels.

About the iBlot 2 Gel Transfer Device
The iBlot 2 Gel Transfer Device is our second-generation dry-transfer device, providing the same speed and convenience as the original iBlot device, but with many new features. The iBlot 2 Gel Transfer Device performs western blotting transfer simply, efficiently, and reliably, within seven minutes or less, and without the need for liquid buffers. The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF).

Specifications

• For Use With (Equipment): Mini Gel Tank
• Membrane Compatibility: Nitrocellulose
• Gel Percentage: 4 to 12%
• No. of Wells: 10-well
• Separation Range: 3.5 to 260 kd
• Thickness (Metric): 1.0 mm

• Gel Size: Mini
• Product Line: Bolt™, iBlot2™
• Gel Type: Bis-Tris Plus
• Quantity: 1 Welcome Pack
• Shipping Condition: Approved for shipment at room temperature or on wet ice

For Research Use Only. Not for use in diagnostic procedures.