Invitrogen™ Human Eotaxin ELISA Kit

Catalog No.	Quantity
KAC2231	96 Tests

Catalog No. KAC2231 Supplier Invitrogen™ Supplier No. KAC2231

Description

ELISA

The Human Eotaxin ELISA quantitates Hu Eotaxin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Eotaxin. Principle of the method The Human Eotaxin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil specific chemokine assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections.

Specifications

• Accession Number: P51671
• Assay Range: 7.8 to 500 pg/mL
• Assay Sensitivity: <2.2 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): Colorimetric Detection
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 6356
• Gene Symbol: Scya11
• Incubation Time: 3 hr.
• Interassay CV: 4.9%
• Intraassay CV: 4.2
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, Biotinylated Detection Antibody, Strepavidin-HRP conjugate, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive plate covers
• Label or Dye: HRP
• Marker Gene: Eotaxin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Eotaxin
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs