Invitrogen™ Human IL-18 ELISA Kit

Description

ELISA

The Human Interleukin 18 (Hu IL-18) ELISA quantitates Hu IL-18 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-18. Principle of the method The Human IL-18 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.

Specifications

• Accession Number: Q14116
• Assay Range: 25.6 to 1000 pg/mL
• Assay Sensitivity: 6.25 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): Colorimetric Detection
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3606
• Gene Symbol: IL18
• Incubation Time: 2.5 hr.
• Interassay CV: 7.5%
• Intraassay CV: 8.3
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Marker Gene: IL-18
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Target: IL-18
• Test Time: 1 hrs 20 mins
• Total Assay Time: 2 hrs 30 mins