Invitrogen™ Human IL1RA ELISA Kit

Description

ELISA

The Human IL-1RA ELISA Kit quantifies human Interleukin 1 Receptor Antagonist (IL-1RA) in serum, plasma, buffered solution, urine or cell culture medium. The assay will recognize both the natural and recombinant IL-1RA. Principle of the method The Human IL-1RA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

Specifications

• Accession Number: P18510
• Assay Range: 31.2 to 2000 pg/mL
• Assay Sensitivity: 4 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant, Urine
• Target Species: Human
• For Use With (Application): Colorimetric
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3557
• Gene Symbol: DIRA,ICIL-1 RA,IL-1 RN,IL-1 ra,IL-1 ra3,IL1F3,IL1RA,IRAP,MVCD4
• Interassay CV: 6.1%
• Intraassay CV: 4.5
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Controls
• Label or Dye: HRP
• Precision: 4 pg/mL
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL, Supernatant 100 μL, Urine 10 μL
• Storage Requirements: 2°C to 8°C
• Target: IL1RA
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 30 mins