Invitrogen™ Human MIP-1a ELISA Kit

Description

ELISA

The Human Macrophage Inflammatory Protein-1 alpha/Chemokine Ligand 3 (Hu MIP-1α/CCL3) ELISA quantitates Hu MIP-1α in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MIP-1α. Principle of the method The Human MIP-1αsolid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.

Specifications

• Accession Number: P10147
• Assay Range: 15.6 to 1000 pg/mL
• Assay Sensitivity: <2 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): Colorimetric
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 6348
• Gene Symbol: G0S19-1,LD78ALPHA,MIP-1-alpha,MIP1A,SCYA3
• Interassay CV: 7.1%
• Intraassay CV: 6.5
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, Biotinylated Detection Antibody, Strepavidin-HRP conjugate, HRP diluent, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive plate covers
• Label or Dye: HRP
• Precision: <2 pg/mL
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: CCL3 (MIP-1 alpha)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs