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Invitrogen™ NativePAGE™ Sample Prep Kit
Description
Includes
1mL of 10% DDM, 1mL of 5% Digitonin, 10mL of NativePAGE, 4X Sample Buffer, 0.5mL NativePAGE 5% G-250 Sample Additive
The kit includes two ready-to-use detergent solutions, 10% DDM (n-dodecyl-β-D-maltoside) and 5% Digitonin, which improve the solubility of hydrophobic and membrane proteins during sample preparation. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE Sample Prep Kit are compatible with NativePAGE Novex Bis-Tris Gels for native electrophoresis, showing increased resolution and reduced streaking.
1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis
Order Info
Shipping Condition: Wet Ice
Specifications
Specifications
| Content And Storage | Contains: • 1 ml of 10% DDM • 1 ml of 5% Digitonin • 10 ml of NativePAGE™ 4X Sample Buffer • 0.5 ml NativePAGE™ 5% G-250 Sample Additive. Store the kit at 2°C to 8°C. |
| Buffer | Sample Loading Buffers |
| Gel Type | Native-PAGE |
| Gel Compatibility | NativePAGE™ Gels |
| Quantity | 1 kit |
| Shipping Condition | Wet Ice |
| Product Line | NativePAGE |
| Product Type | PAGE Sample Prep Kit |
Frequently Asked Questions (FAQs)
We recommend using the NativeMark Unstained Protein Standard, Cat. No. LC0725 for native gel electrophoresis with Tris-Glycine, NuPAGE Tris-Acetate or NativePAGE gels.
We do not recommend using NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels. NuPAGE Invitrogen Bis-Tris gels are optimized for denaturing conditions and have an extremely low operating pH (pH 7.0), which makes it difficult for most proteins to migrate through them in a native state.
NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate or Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.
NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.
NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate gels. When running Tris-Acetate gels under native conditions, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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