Invitrogen™ Nitrocellulose/Filter Paper Sandwich, 0.45 μm, 8.3 x 7.3 cm

Catalog No.	Quantity
LC2001	20 membrane/filter paper sandwiches

Catalog No. LC2001 Supplier Invitrogen™ Supplier No. LC2001

Description

Ideal for blotting proteins and nucleic acids

The 0.45μm pore size is ideal for the transfer of low molecular weight proteins (<20kDa) and nucleic acids (<300bp).

 

• Composed of 100% pure nitrocellulose to provide high-quality transfer
• Contain no support fabrics or detergents
• Are compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, fluorescence, and radiolabeling
• Provide high-sensitivity with low background
• Are supplied in a pre-cut, pre-assembled membrane/filter paper sandwich for convenience
• A protein's properties (i.e., charge, hydrophobicity, etc.) affect its ability to bind to membrane surfaces

 

DNA and RNA Purification and Analysis, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting, Proteins, Expression, Isolation and Analysis, Western Blotting

Specifications

• Length (Metric): 8.3 cm
• Width (Metric): 7.3 cm
• Description: Nitrocellulose Pre-Cut Blotting Membrane, 0.45μm pore size
• Quantity: 20 membrane/filter paper sandwiches
• Format: Sandwich
• Material: Nitrocellulose
• Pore Size: 0.45 μm
• Content And Storage: Store at room temperature.
• Shipping Condition: Room Temperature
• Dimensions (LxW): 7.3 cm x 8.3 cm
• Thickness: 0.8 mm
• Product Line: Novex

Frequently Asked Questions (FAQs)

How should nitrocellulose be treated prior to transferring proteins?

Prewet the nitrocellulose membrane in distilled water or in 1X transfer buffer for 5 min with gentle shaking. This is to prevent any dry spots in the membrane that may interfere with transfer.

How can I store, strip, and reuse my western blot?

For nitrocellulose or PVDF membrane following Western blot detection using a chemiluminescent or fluorescent substrate system: Following transfer, air dry the membrane and place in an envelope, preferably on top of a supported surface to keep the membrane flat. The blot can be stored indefinitely at -80 degrees C. When ready to reprobe, prewet the PVDF blot with alcohol for a few seconds, followed by a few rinses with pure water to reduce the alcohol concentration. Then proceed as normal with blocking step.

FOR STRIPPING/REPROBING OF MEMBRANES: Harsh protocol (see NOTE below for modifications)

1) Submerge the membrane in stripping buffer (100 mM BME, 2% SDS, 62.5 mM Tris-HCl, pH 6.7) and incubate at 50 degrees C for 30 min with occasional agitation. If more stringent conditions necessary, incubate at 70 degrees C.

2) Wash 2 x 10 min in TBS-T/PBS-T at room temperature.

3) Block the membrane by immersing in 5% blocking reagent TBS-T or PBS-T for 1 hr at room temperature.

4) Immunodetection

NOTE: Often you don't need such harsh conditions to remove antibodies from their proteins. The stringency of one or several of the variables can be decreased: lower the temperature, decrease the time, less BME, less SDS, etc. An especially mild but still often effective stripping protocol is lower pH incubation. Example: pH 2.0 Tris 50-100 mM, 30-60 min incubation (you may do two incubations if you wish). Then rinse and block as usual. If you do not wish to re-use the membrane immediately after stripping, you can store the membrane in plastic wrap (wet, you do not want it to dry out). Another simple, mild stripping buffer is 0.1 M glycine•HCl (pH 2.5-3.0), incubation 30 min to 2 hrs room temperature or 37 degrees C, depending on the antibody.

Are your PVDF and nitrocellulose membranes compatible with the Li-COR instrument?

Yes, both our PVDF and nitrocellulose membranes are compatible with the Li-COR instrument.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.