Invitrogen™ Human IR (Phospho) [pY1162/pY1163] ELISA Kit

Catalog No. KHR9131

Supplier: Invitrogen™ KHR9131

Description

ELISA

Kit is designed to detect and quantify the level of IR (Phospho) [pY1162/pY1163] in fresh or frozen human cell lysates. Cross-reactivity with mouse and rat cells has been observed. The assay recognizes both natural and recomb. Human IR (Phospho) [pY1162/pY1163]. Principle of the method A monoclonal capture antibody specific for IR (Phospho) [pY1162/pY1163] has been coated onto the wells of 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a HRP labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

INSR is a receptor tyrosine kinase. Binding of insulin to the extracellular domain of INSR stimulates glucose uptake. INSR and IGF-1 receptors share major structural and functional similarity. The earliest cellular response to insulin stimulation is autophosphorylation of tyrosine in INSR. In humans, the INSR gene is located on chromosome 19. Defects in INSR are the cause of various insulin resistance syndromes and IGF-1R defects may also cause some forms of growth retardation.

Specifications

• Accession Number: P06213,P15127,P15208
• Assay Range: 0.625 to 40 U/mL
• Assay Sensitivity: <0.4 U/mL
• Concentration: 1.6 U/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates
• Substrate Type: Receptor Tyrosine Kinases
• Target Species: Mouse, Rat
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 16337, 24954, 3643
• Gene Symbol: CD220,HHF5
• Incubation Time: 4 hr.
• Interassay CV: 7.6%
• Intraassay CV: 6
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, anti-rabbit-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Marker Gene: IR
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 10 μL
• Storage Requirements: 2°C to 8°C
• Target: INSR
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs