Invitrogen™ Human IR beta ELISA Kit

Catalog No.	Quantity
KHR9111	96 Tests

Catalog No. KHR9111 Supplier Invitrogen™ Supplier No. KHR9111

Description

ELISA

The Human Insulin Receptor beta subunit (Hu IRβ) ELISA quantitates Hu IRβ in human cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Hu IRβ. Cross-reactivity has been observed in mouse and rat cells. Principle of the method The Human IRβ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The insulin receptor. Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor. Defects in IR are the cause of various insulin resistance syndromes and IGF-1R defects may cause some forms of growth retardation. Both these signaling cascades are also important for the development of cancer.

Specifications

• Accession Number: P06213,P15127,P15208
• Assay Range: 0.94 to 60 ng/mL
• Assay Sensitivity: 0.5 ng/mL
• Concentration: 0.94 to 60 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates
• Substrate Type: Receptor Tyrosine Kinases
• Target Species: Mouse, Human, Rat
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 16337, 24954, 3643
• Gene Symbol: CD220,HHF5
• Incubation Time: 4 hr.
• Interassay CV: 8.2%
• Intraassay CV: 5.4
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, anti-rabbit-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Marker Gene: IR
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 10 μL
• Storage Requirements: 2°C to 8°C
• Target: INSR beta
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs