Invitrogen™ SUMO Protease  

A recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae

Manufacturer:  Invitrogen™ 12588018

Catalog No. 12-588-018

Includes: SUMO Protease is provided with 10X high salt and 10X no salt dilution buffers.

Description

SUMO Protease, also known as Ulp, is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence.

• Highly active cleavage
• No non-specific proteolysis (highly specific cleavage)
• Activity from 2°C to 37°
• A six-histidine sequence to facilitate its removal from the digested protein sample

Application:Removal of fusion tags from recombinant proteins
Unit Definition: One unit of SUMO Protease is defined as the amount of enzyme needed to cleave 85% of 2µg of substrate protein at 30°C in one hour
Quality Control: SUMO Protease has greater than 85% single-band purity with no non-specific protease contamination; functionally tested for the absence of any non-specific protease activity

GST-Tagged Protein Purification, His-Tagged Protein Purification, Protein and Peptide Purification, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis

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Order Info

Shipping Condition: Dry Ice

Specifications

• Shipping Condition: Dry Ice
• Species: S. cerevisiae
• Content And Storage: SUMO Protease is provided with 10X high salt and 10X no salt dilution buffers. Store at -80°C. Guaranteed stable for 6 months when properly stored.

For Research Use Only. Not for use in diagnostic procedures.