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Invitrogen™ Novex™ WedgeWell™ Tris-Glycine Welcome Pack and Mini Blot Modules, 4–20%
Description
The Novex Tris-Glcyine Plus Welcome Pack and Mini Blot Modules provide all of the necessary Novex gels and buffers needed to begin using the Mini Gel Tank plus two Mini Blot Modules for wet transfer. The Mini Gel Tank is compatible with all Invitrogen precast and handcast protein gels including Novex gels, NuPAGE gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run or up to two Mini Blot Modules for wet transfer. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use.
The Novex Tris-Glycine Plus Welcome Pack contains:
• Mini Gel Tank (Cat. No. A25977)
• Novex Tris-Glycine Mini Gels, WedgeWell format (2 boxes, 20 gels)
• Novex Tris-Glycine SDS Running Buffer, 10X (Cat. No. LC2675)
• Novex Tris-Glycine SDS Sample Buffer, 2X (Cat. No. LC2676)
• NuPAGE Sample Reducing Agent, 10X (Cat. No. NP0009)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa (Cat. No. 26619)
• 2 x Mini Blot Modules (Cat. No. B1000)
About the Mini Gel Tank
The Mini Gel Tank is compatible with Novex gels, NuPAGE gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.
About Novex Tris-Glycine gels, WedgeWell format
Novex Tris-Glycine mini gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. They enable consistent performance and separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm standard format gels. The wedge-shaped wells also have larger openings, making sample loading easier.
Transfer
For transferring your proteins to a membrane, we recommend using for Novex Tris-Glycine Native Running Buffer (Cat. No. LC2672) or Novex Tris-Glycine Transfer Buffer (Cat. No. LC3675) for traditional wet transfer using the Mini Blot Module (Cat. No. B1000).
About the Mini Blot Module
The Mini Blot Module is a wet transfer device used exclusively with the Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Mini Blot Module is compatible all Invitrogen mini gels (blot size: 8.5 x 8 cm). The blot module accommodates one mini gel per wet transfer. The universal connection and molded gasket make the module easy to use, while the inner core of the module allows for use of less methanol-based transfer buffer per wet transfer (250 mL per gel). At the recommended conditions and constant voltage, proteins can be transferred to nitrocellulose or PVDF membranes typically in 30 to 60 minutes.
Specifications
Specifications
| For Use With (Equipment) | Mini Gel Tank |
| Gel Size | Mini |
| Gel Percentage | 4 to 20% |
| Gel Type | Tris-Glycine |
| Mode of Separation | Denaturing |
| No. of Wells | 15-well |
| Quantity | 1 kit |
| Separation Range | 20 to 200 kDa |
For Research Use Only. Not for use in diagnostic procedures.
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