Novus Biologicals™ pCLNRX Retrovirus Expression Vector

Catalog No.	Quantity
NBP229538	10 μg

Catalog No. NBP229538 Supplier Novus Biologicals™ Supplier No. NBP229538

Description

pCLNRX expression vector is part of RetroMax system designed for maximal virus titer in 293 cells

The pCLNRX expression vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. All members of the RetroMax expression vector family (pCLXSN, pCLNCX, pCLNRX, and pCLNDX) have an extended packaging signal (omega+) and are derived from safety modified retrovirus vectors in which the gag open reading frame has been stopped by a point mutation, thereby minimizing the opportunity for replication competent retrovirus production by recombination with packaging genome.

• RetroMax system takes advantages of two properties of 293 cells (high level of transfectibility and strong E1A-mediated stimulation of CMV promoter controlled transcription)
• 293 cells of nonmurine origin avoid problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells)
• Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells
• Buffer: 10μg in 20μL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)

Recommended Storage

Store at -20°C. Avoid freeze-thaw cycles.

Specifications

• Storage Buffer: 10µg in 20µL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)
• Content And Storage: Store at -20°C. Avoid freeze-thaw cycles.
• Quantity: 10 μg
• Vector: pCLNRX Retrovirus Expression Vector