Invitrogen™ Human NUAK1 (ARK5), His Tag Recombinant Protein

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Recombinant, Full Length

NUAK1 (ARK5) is a serine/threonine kinase that plays a role in many cellular functions. The overexpression of this kinase has been associated with tumor invasion and metastasis (colorectal neoplasm). Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate in transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair.

Specifications

• Accession Number: O60285
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50 mM tris with 0.02% Triton X-100, 0.5 mM EDTA, 150 mM NaCl, 2 mM DTT, 50% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 9891
• Molecular Weight (g/mol): 78.3 kDa
• Name: Human NUAK1 (ARK5), His Tag
• Quantity: 10 μg
• Gene Alias: AMP-activated protein kinase family member 5; AMPK-related protein kinase 5; ARK5; AU014801; AW494241; B230104P22Rik; I79_017442; KIAA0537; NUAK family kinase 1; NUAK family SNF1-like kinase 1; NUAK family, SNF1-like kinase, 1; Nuak1; Omphalocele kinase 1; Omphk1; RGD1309956; ZNUAK family, SNF1-like kinase, 1
• Common Name: ARK5
• KinaseFamily: CAMKL Kinase Family
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Assay Entry: Biochemical binding/kinetic
• Kinase Group: Ser⁄Thr Kinases: CAMK Group
• Shipping Condition: Dry Ice
• System Compatibility: Omnia™ Bulk Substrate
• Target Entry: NUAK1
• Target Gene: NUAK family, SNF1-like kinase, 1
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Full Length
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases
• Research Category: Signal Transduction

Safety and Handling

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