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Invitrogen™ Nuclear Antigen Monoclonal Antibody (235-1)

Description
This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells. This clone stains nuclei of all human cell types giving a diffuse nuclear staining pattern; chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band. The antibody works on acetone-fixed and paraformaldehyde-fixed cells. It is suggested to permeabilize the cells with 0.1-0.4% Triton X-100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.
Specifications
Specifications
| Antigen | Nuclear Antigen |
| Applications | Immunohistochemistry, Immunocytochemistry, Immunohistochemistry (Frozen) |
| Classification | Monoclonal |
| Clone | 235-1 |
| Concentration | 1 mg/mL |
| Conjugate | Unconjugated |
| Formulation | Tissue culture supernatant with no preservative |
| Gene Alias | Histone H1; HNA; HuNu; Nuclei; Nucleus |
| Host Species | Mouse |
| Immunogen | Human cell homogenate. |
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