MP Biomedicals™ Nuclease P1, MP Biomedicals™

Description

Exhibits both phosphodiesterase and monoesterase activities. Nuclease P1, MP Biomedicals is a single strand-specific nuclease that exhibits high selectivity for single-stranded nucleic acids and single-stranded regions in double-stranded nucleic acids. De

Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phosphooligonucleotides. It does not appreciably degrade double-stranded nucleic acids, especially in the presence of more than 400 mM sodium chloride at pH 6.0.

• Useful at removing single stranded strands hanging off the end of double stranded DNA and at completely cleaving melted DNA for simple DNA composition analysis
• Improves the sensitivity of a 32P-labeling method for the detection of DNA adducts
• Used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent
• Used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5′ or 3′ end group labeled RNA

Specifications

• Color: White
• pH: Optimum pH: 5.3 (RNA and heat-denatured DNA) and 7.2 (3′-AMP) (Lit.)
• Content And Storage: 4°C
• Quantity: 1 Vial
• Form: Solid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov