Nucleolin/NCL Rabbit anti-Human, Mouse, Polyclonal, Bethyl Laboratories

Catalog No.	Quantity
50-155-8275	100 μL

Catalog No. 50-155-8275 Supplier Bethyl Laboratories, Inc Supplier No. A300711A

Description

Rabbit Polyclonal Antibody

The recommended shelf life for this product is 1 year from date of receipt. Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Based on 100% sequence identity, this antibody is predicted to react with Orangutan

Nucleolin, which is identical to human DNA helicase IV, is a major nucleolar phosphoprotein which is associated with preribosomal RNA and is implicated in the early stage of preribosomal RNP assembly and processing. This 100 kDa protein has three major domains: a N-terminal domain comprised of long acidic stretches interspersed with basic repeats, similar to the structure of a high mobility group-type protein, a central domain that contains four RNA binding elements, a C-terminal domain approximately 85 amino acids long that is rich in glycine, arginine, and phenylalanine residues. Nucleolin fluctuates in parallel to DNA synthesis; intact 100 kDa protein is the major species in actively dividing cells, whereas the degraded forms are relativley abundant in nondividing cells. Nucleolin can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes. Nucleolin also interacts directly with DNA topoisomerase I. It is located mainly in dense fibrillar regions of the nucleolus. Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. It interacts with APTX and contains 4 RNA recognition motif (RRM) domains.

Specifications

• Antigen: Nucleolin/NCL
• Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Concentration: 0.2 mg/ml
• Conjugate: Unconjugated
• Formulation: TBS with 0.1% BSA and 0.09% sodium azide
• Gene: NCL
• Gene Accession No.: P09405, P19338
• Gene Alias: C23, FLJ45706, FLJ59041
• Gene Symbols: NCL
• Host Species: Rabbit
• Immunogen: Between 550 and C-term
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 17975, 4691
• Target Species: Human, Mouse
• Content And Storage: 4° C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG