Thermo Scientific™ Nunc™ Cell Culture Inserts in Carrier Plate Systems

Catalog No.	Pore Size	No. of Wells
12-567-204	3 μm	12
12-567-202	0.4 μm	24
12-567-203	3 μm	24
12-567-205	8 μm	24
12-567-207	No Inserts	24
12-567-206	8 μm	12
12-567-208	No Inserts	12
12-567-201	0.4 μm	12

Catalog No. 12-567-204 Supplier Thermo Scientific™ Supplier No. 141080

Description

Use up to 24 inserts at a time with Thermo Scientific™ Nunc™ Carrier Plate for Cell Culture Inserts, for individual compartment cultures or cultures with shared media.

The Thermo Scientific™ Nunc™ Carrier Plate system is designed to enable three different hanging height adjustments for the Cell Culture Inserts to accommodate liquid-air-interface culture and various cell feeding protocols. Individually packed, the multi-well Carrier Plate system comes with or without the Cell Culture Inserts.

Features:

• Meets ANSI™ standards
• Equipped with alphanumeric well identification
• Polycarbonate membrane is available in three pore sizes for enhanced flexibility in your research.
• Inserts are ideal for for 3-D imaging; translucent membrane becomes transparent when wet.
• Carrier plates come in two formats that correspond with our 12- and 24-well Thermo Scientific™ Nunclon™ Delta-treated dishes.
• Uniquely engineered carrier plate features a support structure that enables variable height.
• Simply rotate the insert to engage one of the three height-adjusting tabs into the carrier plate supports.

Specifications

• Inserts per Plate: 12
• Culture Area: 1.13 cm²
• Material: Polycarbonate (Membrane)
• Volume (Metric) Working: 2 mL (Low Hanging Position), 3 mL (Medium Hanging Position), 4 mL (High Hanging Position)
• Pore Density: <1.7 x 10⁶ pores/cm²
• Pore Size: 3 μm
• Type: Carrier Plate with Cell Culture Inserts
• For Use With: Cell Culture Insert
• No. per Pack: 1
• No. per Case: 4
• Dish Type: Carrier Plate Systems
• Color: Clear
• Surface Treatment: Cell Culture
• Sterility: Sterile
• No. of Wells: 12

Frequently Asked Questions (FAQs)

What is the difference between the 24-well plate format for the Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates (Cat. No. 140620) and the 24-well plate format for the Nunc Cell Culture Inserts in Carrier Plate Systems (Cat. No. 141002)?

The difference between the two 24-well plate formats is as follows:

• Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates (Cat. No. 140620): There are only 12 inserts in the 24-well multi-well plate, leaving every second column free without an insert. This is a preferred configuration for some co-culture experiments, as it makes it easy to access the culture grown in the multi-well plate for media changes, etc., by placing the insert in the empty well next to it. The inserts are placed directly into the wells here, leaving a 0.9 mm gap between the bottom of the well and the insert membrane.
• Nunc Cell Culture Inserts in Carrier Plate Systems (Cat. No. 141002): There are 24 inserts in the 24-well carrier plate. The carrier plate with the hanging slots allows for adjustment of the insert height. The three hanging positions in the carrier plate allow for distance of approximately 0.9 mm, 3.3 mm, or 6.3 mm between the well-bottom and insert growth surface for both 12- and 24-well plate formats. This configuration is preferred when customer needs more versatility, for example to facilitate air-liquid interface culture, and other co-culture applications.

Note: details on plate configuration can also be found within the following product brochure (pg 3).

With the Nunc Polycarbonate Cell Culture Inserts in Multi-Well Plates, what is the distance between the cell culture membrane and the bottom of the multi-well dish?

The membrane is approximately 1.0 mm above the bottom of the dish for the Nunc Polycarbonate Cell Culture Inserts.

Can I observe cells on the cell culture membrane when the Nunc Polycarbonate Cell Culture Insert is inside of a multi-well plate?

Due to the large pore size of the Nunc polycarbonate inserts, spots or holes are observed and they represent the membrane. In this plane of focus, the most visible cells are the ones located on the membrane, whereas the slightly blurred backdrop are the cells on the dish that are in a different plane of focus. These cells do not photograph with great resolution due to the slightly opaque quality of the membrane. However, these cells are visible in culture.

What is the recommended Multidish chamber media volume per carrier plate insert and height position?

Low position: 2 mL (12-well), 1 mL (24-well), Medium position: 3 mL (12-well), 1.5 mL (24-well), High position: 4 mL (12-well), 2 mL (24-well). Some optimization may be necessary for development purposes.

What is the difference between your cell culture membrane inserts and carrier plates?

Standard culture membrane inserts are placed directly into the wells of a Multidish, and the feet at the bottom of the insert maintain a distance of about 1 mm between the membrane and the Multidish culture surface. The “carrier plate” culture membrane product adds a tray between the Multidish and the lid, with a set of hanger brackets at each well. These brackets engage one of three sets of tabs on each insert, to allow for selection between 3 different distances (approximately 1, 3, or 6 mm) between the membrane and the Multidish culture surface. This can allow for more flexibility in experiments, such as distance-dependent interaction between populations or media volume under cultures at the air-media interface.

Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.