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Thermo Scientific™ Nunc™ Cell Culture/Petri Dishes
Description
With wide selections in size and style, Thermo Scientific Nunc cell culture dishes suit a multitude of experimental needs in your cell culture routine. Perform a variety of applications in dishes with the Nunclon™ Delta surface treatment that promotes maximum adhesion for a broad range of cell types.
Features
- Top and bottom stacking rings for easier handling and more stable stacking
- Sterile and non-pyrogenic
- Available with or without air vents for gas exchange
- 2 x 2 mm grid for cloning experiments and determination of plating efficiency
Includes: a certificate that assures user of biological performance as well as traceability
Warranty: 90 days
Additional information
- Cat. Nos. 174926 and 169558 feature a 2 x 2 mm grid
- Cat. No. 171099 is for suspension cell culture; it is not Nunclon Delta treated
Specifications
Specifications
| Closure Color | Clear |
| Closure Included | Closure Included |
| Closure Material | Polystyrene |
| Bottom Shape | Flat |
| Capacity (Metric) | 3 mL (Well) |
| Color | Clear |
| Culture Area | 8.8 sq. cm |
| Gridded | Gridded |
| Material | Polystyrene |
| Shape | Round |
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Frequently Asked Questions (FAQs)
This depends largely on the cell line and working conditions (considerations include headspace for gas exchange, supplements used, and optimal feeding schedule, among others) but we do offer guidelines for development purposes (0.3 to 0.5 mL per cm2) . Note that these volumes are approximate and may have to be modified for best performance. T25 Flask = 7 mL, T75 Flask = 25 mL, T175 Flask = 55 mL, T225 Flask = 70 mL, 300cm2 Flask = 100 mL, 500 cm2 TripleFlask = 200 mL, 35 mm round culture dish = 3 mL, 60 mm round culture dish = 5 mL, 100 mm round culture dish = 12 mL, 150 mm round culture dish = 35 mL, 4 (rectangular) well Multidish = 5 mL, 8 (rectangular) well Multidish = 3 mL, 4 (round) well Multidish = 1 mL, 6 well Multidish = 3 mL, 12 well Multidish = 2 mL, 24 well Multidish = 1 mL, 48 well Multidish = 0.5 mL.
Nunclon Delta refers to our performance-certified surface for culture of adherent cells, available in a variety of common formats (flasks, plates, multiwell dishes). It is tested with at least four cell lines (varies by product and manufacturing location) commonly used in research, and it should work well for most robust cell lines. It does not involve addition of coating reagent, but rather is manufactured by exposure of the molded products to a proprietary energy source, which renders the surface more hydrophilic by means of increasing the concentration of oxygen-containing (e.g. hydroxyl) molecular groups. It is by this hydrophilic property that anchorage-dependent cells are able to attach to the surface and become confluent.
Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.
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