Thermo Scientific™ Nunc™ Cell Culture/Petri Dishes

Catalog No.	Culture Area	Surface Treatment	Gridded	Vent
12-565-94	21.5 sq. cm	Nunclon Delta	Not Gridded	Lid without airvent
12-565-90	8.8 sq. cm	Nunclon Delta	Not Gridded	Lid without airvent
12-565-91	8.8 sq. cm	Nunclon Delta	Not Gridded	Lid with airvent
12-565-92	8.8 sq. cm	Nunclon Delta	Gridded	Lid with airvent
12-565-93	8.8 sq. cm	Non-treated	Not Gridded	Lid with airvent
12-565-97	20.8 sq. cm	Cell Culture	Not Gridded	Lid with airvent
12-565-95	21.5 sq. cm	Nunclon Delta	Not Gridded	Lid with airvent
12-565-96	21.5 sq. cm	Nunclon Delta	Gridded	Lid with airvent
12-565-020	56.7 sq. cm	Nunclon Delta	Not Gridded	Lid with airvent
12-565-100	145 sq. cm	Nunclon Delta	Not Gridded	Lid with airvent
12-567-650	56.7 sq. cm	Nunclon Delta	Not Gridded	Lid with airvent
12-565-352	150 sq. cm	Nunclon Delta	Not Gridded	Lid with airvent

Catalog No. 12-565-94 Supplier Thermo Scientific™ Supplier No. 150326

Description

Perform a variety of applications with cell culture/Petri dishes offered in a broad range of sizes and styles to suit a variety of experimental needs.

With wide selections in size and style, Thermo Scientific Nunc cell culture dishes suit a multitude of experimental needs in your cell culture routine. Perform a variety of applications in dishes with the Nunclon™ Delta surface treatment that promotes maximum adhesion for a broad range of cell types.

Features

• Top and bottom stacking rings for easier handling and more stable stacking
• Sterile and non-pyrogenic
• Available with or without air vents for gas exchange
• 2 x 2 mm grid for cloning experiments and determination of plating efficiency

Includes: a certificate that assures user of biological performance as well as traceability

Warranty: 90 days

Additional information

• Cat. Nos. 174926 and 169558 feature a 2 x 2 mm grid
• Cat. No. 171099 is for suspension cell culture; it is not Nunclon Delta treated

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

• Closure Color: Clear
• Closure Included: Closure Included
• Closure Material: Plastic, Polystyrene
• Bottom Shape: Flat
• Capacity (Metric): 5 mL (Well)
• Color: Clear
• Culture Area: 21.5 sq. cm
• Gridded: Not Gridded
• Material: Polystyrene
• Shape: Round
• Sterility: Sterile
• Surface Treatment: Nunclon Delta
• Type: Cell Culture Dish
• Volume (Metric) Working: 5 mL
• Well Count: 1
• Includes: Without Airvent
• No. per Case: 400/Cs.
• No. per Pack: 10 Pk.
• Quantity: 10 Pk., 400/Cs.
• Vent: Lid without airvent
• Height (Metric): 15 mm
• Diameter (Metric): 60 mm

Frequently Asked Questions (FAQs)

What is the difference between the Nunclon Delta surface and a tissue culture (TC) surface from a competitor?

Our Nunclon Delta surface for adherent cells is a standard tissue culture (TC) surface modification that makes the polystyrene surface more hydrophilic. This facilitates maximum adhesion for a broad range of cell types.

The Nunclon Delta surface is Thermo Scientific Nunclon Delta-certified, which is a Thermo Fisher proprietary certification that has a quality standard to help ensure consistency. Every lot of Thermo Scientific Nunclon Delta-certified cell cultureware is tested with Gibco media for:
- Less than 10% growth variation in L929, Hel299 and primary chick embryo cells
- Less than 15% variation colony formation of V79-4 cells

More information and all applicable products can be found in the "Product selection guide for every step of your cell culture workflow" brochure .

What is the recommended working volume of Nunc adherent cell culture products?

This depends largely on the cell line and working conditions (considerations include headspace for gas exchange, supplements used, and optimal feeding schedule, among others) but we do offer guidelines for development purposes (0.3 to 0.5 mL per cm2) . Note that these volumes are approximate and may have to be modified for best performance. T25 Flask = 7 mL, T75 Flask = 25 mL, T175 Flask = 55 mL, T225 Flask = 70 mL, 300cm2 Flask = 100 mL, 500 cm2 TripleFlask = 200 mL, 35 mm round culture dish = 3 mL, 60 mm round culture dish = 5 mL, 100 mm round culture dish = 12 mL, 150 mm round culture dish = 35 mL, 4 (rectangular) well Multidish = 5 mL, 8 (rectangular) well Multidish = 3 mL, 4 (round) well Multidish = 1 mL, 6 well Multidish = 3 mL, 12 well Multidish = 2 mL, 24 well Multidish = 1 mL, 48 well Multidish = 0.5 mL.

What is Nunclon Delta?

Nunclon Delta refers to our performance-certified surface for culture of adherent cells, available in a variety of common formats (flasks, plates, multiwell dishes). It is tested with at least four cell lines (varies by product and manufacturing location) commonly used in research, and it should work well for most robust cell lines. It does not involve addition of coating reagent, but rather is manufactured by exposure of the molded products to a proprietary energy source, which renders the surface more hydrophilic by means of increasing the concentration of oxygen-containing (e.g. hydroxyl) molecular groups. It is by this hydrophilic property that anchorage-dependent cells are able to attach to the surface and become confluent.

Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.