Thermo Scientific™ Nunc™ TripleFlask Treated Cell Culture Flasks

Catalog No.	Surface Treatment	Closure Type	Barcode
12-566-405	Cell Culture-treated	Vent/Close	No
12-565-24	Cell Culture-treated	Solid	No
12-565-25	Cell Culture-treated	Filter	No
12-565-541	Cell Culture-treated	Filter	Yes
12-566-410	Nunclon Delta	Filter	Yes

Catalog No. 12-566-405 Supplier Thermo Scientific™ Supplier No. 132865

Description

Perform large cell mass applications, such as drug screening and scale up, with these cell-culture treated TripleFlask cell culture flasks.

Perform cell culture expansion and experience 500 cm² total growth area within the footprint of a standard flask using Thermo Scientific Nunc TripleFlask Treated Cell Culture Flasks. Total culture area is achieved by way of the three parallel layers and a straight neck design that works effectively within automation systems. These flasks are subjected to Nunclon Delta treatment, a proprietary cell culture surface treatment that offers maximum adhesion for a broad range of cell types.

Three layers of growth surface provide a total culture area of 500 cm² in the same footprint as a standard Nunc 175 cm² flask.

• Recommended working volume is 200 mL
• Promotes monolayer adherent cell culture
• Also available barcoded with large Code 128
• Nunclon Delta Certificate included in each case for monolayer formation, cloning efficiency, non-cytotoxic, non-pyrogenic, and sterility

Warranty: 90 days

Specifications

• Barcode: No
• Description: Nunc Cell Culture Treated TripleFlask, Vent/Close Cap
• Capacity (Metric): Disc
• Material: Polystyrene
• Product Type: Flask
• Disposable: Single-use
• Graduated: Yes
• Sterility: Sterile
• Bottom Shape: Flat
• Closure Type: Vent/Close
• Culture Area: 500 cm²
• Surface Treatment: Cell Culture-treated
• Volume (Metric) Working: 200 mL

Frequently Asked Questions (FAQs)

What is the recommended working volume of Nunc adherent cell culture products?

This depends largely on the cell line and working conditions (considerations include headspace for gas exchange, supplements used, and optimal feeding schedule, among others) but we do offer guidelines for development purposes (0.3 to 0.5 mL per cm2) . Note that these volumes are approximate and may have to be modified for best performance. T25 Flask = 7 mL, T75 Flask = 25 mL, T175 Flask = 55 mL, T225 Flask = 70 mL, 300cm2 Flask = 100 mL, 500 cm2 TripleFlask = 200 mL, 35 mm round culture dish = 3 mL, 60 mm round culture dish = 5 mL, 100 mm round culture dish = 12 mL, 150 mm round culture dish = 35 mL, 4 (rectangular) well Multidish = 5 mL, 8 (rectangular) well Multidish = 3 mL, 4 (round) well Multidish = 1 mL, 6 well Multidish = 3 mL, 12 well Multidish = 2 mL, 24 well Multidish = 1 mL, 48 well Multidish = 0.5 mL.

Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.