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Invitrogen™ O-GlcNAc Monoclonal Antibody (RL2), PE, eBioscience™, Invitrogen™
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Catalog No. 12979342
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100 Tests
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12-979-342 100 Tests
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Catalog No. 12-979-342 Supplier Invitrogen™ Supplier No. 12979342
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Description

Mouse Monoclonal Antibody

Description: This RL2 monoclonal antibody recognizes proteins with O-N-acetylglucosamine (O-GlcNAc) glycosylation. It was originally developed by immunizing mice with the rat liver nuclear envelopes containing nuclear pore complexes. The RL2 clone has been successfully used in Western blot, immunofluorescence, immunoprecipitation, and flow cytometry in a wide variety of mammalian cells. Applications Reported: This RL2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RL2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

O-linked N-acetylglucosamine (O-GlcNAc) is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine or threonine residues. Unlike other protein glycosylations, O-GlcNAc modifications occur within the nucleus and cytoplasm. They are found on many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins. O-GlcNAc glycosylations are thought to obscure phosphorylation sites, counteracting phosphorylation-dependent signaling pathways and protein interactions.
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Specifications

Antigen O-GlcNAc
Applications Flow Cytometry
Classification Monoclonal
Clone RL2
Concentration 5 μL/Test
Conjugate PE
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene OGT
Gene Accession No. O15294
Gene Alias GlcNAc; HINCUT-1; HRNT1; O-GLCNAC; O-GlcNAc transferase p110 subunit; O-GlcNAc transferase subunit p110; OGT; O-linked N-acetylglucosamine (GlcNAc) transferase; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); O-linked N-acetylglucosamine transferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
Gene Symbols OGT
Host Species Mouse
Immunogen Rat Liver nuclear envelopes.
Purification Method Affinity chromatography
Quantity 100 Tests
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 8473
Target Species Chemical
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG1 κ
Target Molecule Chemical
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov
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