Invitrogen™ O-linked N-acetylglucosamine (O-GlcNAc) Monoclonal Antibody (RL2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1072

Catalog No. MA1072

Description

MA1-072 detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, virus and rat tissues. MA1-072 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes up to eight different proteins from the NPC of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC. Immunofluorescence staining of the NPC with MA1-072 primarily labels NPC O-linked glycoproteins and has been successfully used on a wide variety of mammalian cells. Labeling occurs at the NPC, with most of the labeling at the cytoplasmic and/or nucleoplasmic margins, as well as within the nucleus. The MA1-072 immunogen is pore complex-lamina fraction purified from rat liver nuclear envelopes.

O-linked N-acetylglucosamine (O-GlcNAc) is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine or threonine residues. Unlike other protein glycosylations, O-GlcNAc modifications occur within the nucleus and cytoplasm. They are found on many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins. O-GlcNAc glycosylations are thought to obscure phosphorylation sites, counteracting phosphorylation-dependent signaling pathways and protein interactions.

Specifications

• Antigen: O-linked N-acetylglucosamine (O-GlcNAc)
• Classification: Monoclonal
• Concentration: 2 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: O15294, Q8CGY8, P56558
• Gene Symbols: OGT
• Immunogen: Pore complex-lamina fraction purified from rat liver nuclear envelopes.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG1

• Applications: ChIP Assay, Dot Blot, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: RL2
• Conjugate: Unconjugated
• Gene: OGT
• Gene Alias: GlcNAc; HINCUT-1; HRNT1; O-GLCNAC; O-GlcNAc transferase p110 subunit; O-GlcNAc transferase subunit p110; OGT; O-linked N-acetylglucosamine (GlcNAc) transferase; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); O-linked N-acetylglucosamine transferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 108155, 26295, 8473
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid
• Target Molecule: Chemical