Invitrogen™ O-linked N-acetylglucosamine (O-GlcNAc) Monoclonal Antibody (RL2)

Catalog No.	Quantity
MA1072	100 μL

Catalog No. MA1072 Supplier Invitrogen™ Supplier No. MA1072

Description

Mouse Monoclonal Antibody

MA1-072 detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, virus and rat tissues. MA1-072 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes up to eight different proteins from the NPC of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC. Immunofluorescence staining of the NPC with MA1-072 primarily labels NPC O-linked glycoproteins and has been successfully used on a wide variety of mammalian cells. Labeling occurs at the NPC, with most of the labeling at the cytoplasmic and/or nucleoplasmic margins, as well as within the nucleus. The MA1-072 immunogen is pore complex-lamina fraction purified from rat liver nuclear envelopes.

O-linked N-acetylglucosamine (O-GlcNAc) is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine or threonine residues. Unlike other protein glycosylations, O-GlcNAc modifications occur within the nucleus and cytoplasm. They are found on many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins. O-GlcNAc glycosylations are thought to obscure phosphorylation sites, counteracting phosphorylation-dependent signaling pathways and protein interactions.

Specifications

• Antigen: O-linked N-acetylglucosamine (O-GlcNAc)
• Applications: ChIP Assay, Dot Blot, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: RL2
• Concentration: 2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% sodium azide
• Gene: OGT
• Gene Accession No.: O15294, Q8CGY8, P56558
• Gene Alias: GlcNAc; HINCUT-1; HRNT1; O-GLCNAC; O-GlcNAc transferase p110 subunit; O-GlcNAc transferase subunit p110; OGT; O-linked N-acetylglucosamine (GlcNAc) transferase; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); O-linked N-acetylglucosamine transferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
• Gene Symbols: OGT
• Host Species: Mouse
• Immunogen: Pore complex-lamina fraction purified from rat liver nuclear envelopes.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 108155, 26295, 8473
• Target Species: Chemical
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1
• Target Molecule: Chemical