Invitrogen™ Occludin Polyclonal Antibody

Catalog No.	Quantity
40-470-0	100 μg

Catalog No. 40-470-0 Supplier Invitrogen™ Supplier No. 404700

Description

Rabbit Polyclonal Antibody

This antibody is specific for the human occludin protein. On western blots, it identifies the target band at ~65 kDa. Reactivity has been confirmed with human Caco-2 and HT29, dog MDCK, mouse TCMK, and rat KNRK cell lysates, and mouse kidney, liver, and rat liver homogenates by western blotting, Caco-2 cells by immunofluorescence, and paraffin-embedded human small intestine tissue by immunohistochemistry. For immunofluorescence using Caco-2 cells, fixation with cold ethanol is recommended. For immunohistochemistry in paraffin-embedded tissues, enzyme digestion with pepsin is required prior to staining.

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

Specifications

• Antigen: Occludin
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.25 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.1% sodium azide; pH 7.4
• Gene: OCLN
• Gene Accession No.: Q16625, Q28269, Q61146, Q6P6T5
• Gene Alias: AI503564; BLCPMG; Occludin; occludin 1B; Ocl; Ocln; phosphatase 1, regulatory subunit 115; PPP1R115; tight junction protein occludin; tight junction structural protein
• Gene Symbols: OCLN
• Host Species: Rabbit
• Immunogen: Synthetic peptide derived from the C-terminal region of the human occludin protein.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100506658, 18260, 403844, 83497
• Target Species: Canine, Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov