Thermo Scientific Chemicals Oil Red O

Catalog No.	Quantity
AAA1298922	100 g
AAA1298914	25 g

Catalog No. AAA1298922 Supplier Thermo Scientific Chemicals Supplier No. A1298922

Description

CAS: 1320-06-5 | C26H24N4O | 408.51 g/mol

Oil Red O is suitable as a lipid and lipoprotein stain on cellulose acetate. It is one of the diazo dyes used for staining of neutral triglycerides and lipids. It is used to analyze muscle fibers. It acts as an agent that stains lipids, muscle fibers and alveolar macrophages. Further, it is also used as marking dye. It is an active component of red colored smokes in pyrotechnics.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Oil Red O is suitable as a lipid and lipoprotein stain on cellulose acetate. It is one of the diazo dyes used for staining of neutral triglycerides and lipids. It is used to analyze muscle fibers. It acts as an agent that stains lipids, muscle fibers and alveolar macrophages. Further, it is also used as marking dye. It is an active component of red colored smokes in pyrotechnics.

Solubility
Soluble in ethanol:chloroform (1:1). Insoluble in water.

Notes
Incompatible with strong oxidizing agents.

Specifications

Chemical Identifiers

• CAS: 1320-06-5
• Molecular Formula: C26H24N4O
• Molecular Weight (g/mol): 408.51
• MDL Number: MFCD00003898
• InChI Key: HJAYEODIXUYVIC-LTFAAIOASA-N
• Synonym: C.I. 26125; Solvent Red 27
• PubChem CID: 6046885
• IUPAC Name: (1Z)-1-[[4-[(2,5-dimethylphenyl)diazenyl]-2,5-dimethylphenyl]hydrazinylidene]naphthalen-2-one
• SMILES: CC1=CC=C(C)C(=C1)N=NC1=CC(C)=C(N\N=C2/C(=O)C=CC3=CC=CC=C23)C=C1C

• Melting Point: ∼166°C (decomposition)
• Beilstein: 3491382
• Solubility Information: Soluble in ethanol:chloroform (1:1). Slightly soluble in water.
• Formula Weight: 408.51
• Quantity: 100 g
• Chemical Name or Material: Oil Red O

Frequently Asked Questions (FAQs)

How do I prepare a stock solution and working solution of Oil Red O for staining?

We recommend the following instructions to prepare a stock or working solution of Oil Red O:

Oil Red O Stock Solution
1. Dissolve 300 mg of Oil Red O in 100 mL of 100% isopropanol.
2. Mix well and leave undisturbed for 20 minutes to make the Oil Red O Stock Solution.
The Oil Red O stock solution is stable for 1 year.

Oil Red O Working Solution
1. Add 3 parts of Oil Red O Stock Solution (30 mL) to 2 parts of water (20 mL).
2. Mix well and leave undisturbed for 10 minutes.
3. Filter through Whatman No. 1 filter paper.
The Oil Red O working solution is stable for 2 hours and must be prepared 15 minutes before use.

Safety and Handling

EINECSNumber	215-295-3
TSCA	Yes
Recommended Storage	Ambient temperatures

SDS

RUO – Research Use Only