Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Flow Cytometry Products
  5. Flow Cytometry Compensation, Counting and Calibration
  6. Invitrogen™ OncoStat Panel, SNAP-ChIP™ Spike-in 

Invitrogen™ OncoStat Panel, SNAP-ChIP™ Spike-in

Catalog No. A47355
Encompass_Preferred
Change view
Click to view available options
Quantity:
100 reactions
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
A47355 100 reactions
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. A47355 Supplier Invitrogen™ Supplier No. A47355
Only null left
Add to Cart
Add to Cart

Description

SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation) uses DNA-barcoded semi-synthetic nucleosomes bearing distinct histone mutations as spike-in ChIP controls.

  • SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation) uses DNA-barcoded semi-synthetic nucleosomes bearing distinct histone mutations as spike-in ChIP controls.
  • Homogenous, fully defined standards faithfully represent target mononucleosomes in the experimental sample
  • Nucleosomes are subjected to rigorous quality control for lot-to-lot consistency
  • Unique DNA barcodes can be distinguished from experimental sample genomes
  • Spike-ins provide a direct readout of antibody performance in ChIP
  • Ability to monitor technical variability between samples
  • Defined standards enable universal normalization across experiments
  • SNAP-ChIP panels are directly compatible with your current ChIP workflow, with semi-synthetic nucleosomes bearing the mutation of interest immunoprecipitated and processed alongside sample chromatin.
  • Users can directly monitor antibody capability (specificity and enrichment) in a ChIP experiment by measuring the recovery of on- and off-target nucleosomes. The SNAP spike-ins can be further used as a defined standard to normalize your ChIP data, thereby controlling for technical variability across experiments.
  • The OncoStat Panel consists of a pool of nucleosomes harboring seven well-studied histone H3.3 mutations that have been implicated in cancer (histone H3.3K4M, H3.3K9M, H3.3K27M, H3.3G34R, H3.3G34V, H3.3G34W and H3.3K36M) plus a wild-type H3.3 control. Further, each of these eight nucleosomes is wrapped by two independent DNA barcodes (16 total barcodes in the panel), allowing for an internal technical replicate in each ChIP reaction.
  • A single spike-in of the panel allows users to check antibody specificity by examining the post-IP recovery of on- versus off-target SNAP-ChIP nucleosomes, supporting the generation of high quality ChIP data.
TRUSTED_SUSTAINABILITY

Specifications

Description Recombinant Nucleosome Panel
Content And Storage Store at -5 to -30°C.
No. of Tests 100 Reactions
Quantity 100 reactions
Product Line OncoStat, SNAP-ChIP
Product Type SNAP-ChIP Panel

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.