Invitrogen™ Opsin 4 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1781

Catalog No. PA1-781

Description

PA1-781 detects rat melanopsin. PA1-781 has been successfully used in Western blot, immunofluorescence, and immunocytochemistry procedures. By Western blot, this antibody detects ~53 and ~85 kDa proteins representing overexpressed unglycosylated and glycosylated melanopsin from HEK 293 cells (does not work well on endogenously expressed protein). By immunofluorescence and immunocytochemistry, PA1-781 detects melanopsin from rat retinal ganglion cells (RGCs). The PA1-781 immunogen is a synthetic peptide corresponding to residues E(455) Q K S K T P K T K R H L P S L D R R M(474) of rat melanopsin.

Melanopsin is an opsin-like protein found in the retinal ganglion cells of mammals. Melanopsin is believed to be part of a secondary optical system that parallels that of the classic rod and cone system. This second system reports directly to the suprachiasmatic nucleus and is responsible for regulation of circadian rhythms. Melanopsin is believed to be the primary photopigment responsible for the regulation of these circadian rhythms, and Melanopsin knockout mice have been generated which demonstrate decreased capacity to entrain to light and dark cycles.

Specifications

• Antigen: Opsin 4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: OPN4
• Gene Alias: 1110007J02Rik; Gm533; LOC721663; Melanopsin; MGC142118; MOP; Mopn; OPN 4; OPN4; opn4 {ECO:0000312; opsin 4; opsin 4 (melanopsin); opsin-4; RGD:621701}
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 192223
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: Q8R456
• Gene Symbols: OPN4
• Immunogen: Synthetic peptide corresponding to residues E(455) Q K S K T P K T K R H L P S L D R R M(474) of rat melanopsin.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Rat
• Product Type: Antibody
• Isotype: IgG