OPTIZYME™ DNase I (RNase-Free), Fisher BioReagents™

Description

OPTIZYME™ DNase I is a recombinant endonuclease that digests DNA and is RNase-Free.

Applications and Features:

• OPTIZYME DNase I, RNase-Free is used for the preparation of DNA-free RNA
• Removal of template DNA following in vitro transcription
• DNA labeling by nick translation in conjunction with DNA Polymerase I
• Preparation of DNA-free RNA prior to RT-PCR
• Studies of DNA-protein interactions by DNase I, RNase-free footprinting
• Enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+or Mn2+ions
• Purified from non-animal host with a lower level of intrinsic RNases

Supplied with:

• 10X OPTIZYME DNase I Buffer: 100mM Tris-HCl (pH 7.5 at 25°C), 25mM MgCl₂, 1mM CaCl₂
• 50mM OPTIZYME EDTA (for inactivation by heat at 65°C for 10min)

Source: E. Coli cells with a cloned gene encoding bovine DNase I
Quality Control:

• Ribonuclease-free
• Functionally tested for the digestion of template DNA after in vitro transcription

Specifications

• Concentration: 1 U/μL
• Color: Colorless
• pH: 7.5
• For Use With (Application): Preparation of DNA-free RNA, removal of template DNA following in-vitro transcription, DNA labeling by nick translation in conjunction with DNA Polymerase I, Preparation of DNA-free RNA prior to RT-PCR, Studies of DNA-Protein interactions by DNase I, RNase-free footprinting
• Storage Buffer: OPTIZYME™ DNase I Buffer 10X : Tris HCl 100mM (7.5 pH at 25°C), MgCl2 25mM, CaCl2 1mM, OPTIZYME™ EDTA 50mM (for inactivation by heat at 65°C for 10min.)
• Content And Storage: -20°C
• Source: E. coli cells with a cloned gene encoding bovine DNase I
• Quantity: 1000 U
• Product Type: DNase I (RNase-free)
• Form: Liquid

Safety and Handling

SDS