OPTIZYME™ MspI (HpaII), Fisher BioReagents™

Description

5'...C^C G G...3'
3'...G G C^C...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

• 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
• Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

• Buffer 1: 50 - 100%
• Buffer 2: 50 - 100%
• Buffer 3: 0 - 20%
• Buffer 4: 100%
• Buffer 5: 0 - 20%

Storage Buffer:

• 10mM potassium phosphate (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with MspI.

Note:

• When the external C in the sequence: CCGG is methylated, both MspI and HpaII cannot cleave. MspI is an isoschizomer of HpaII. However, unlike HpaII, MspI can cleave the sequence when the internal C residue is methylated.

Compatible Ends: Bsp119I, Bsu15I, Hin1I, Hin6I, MaeII, NarI, Psp1406I, SsiI, TaqI, XmiI

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: Never overlaps - no effect

CpG: Completely overlaps - no effect

• CCGG

(No effect on cleavage)

EcoKI: Never overlaps - no effect

EcoBI: Never overlaps - no effect

Specifications

• Concentration: 10 U/μL
• Components: 10X OPTIZYME™ Buffer 4
• Incubator Temperature: 37°C
• pH: 7.4
• For Use With (Application): Gene cloning and DNA genotyping
• Storage Buffer: 10mM Potassium Phosphate (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
• Quantity: 3000 U
• Cut Site: C.CGG
• Product Type: MspI
• Form: Liquid

Safety and Handling

SDS